Super-Resolved Traction Force Microscopy (STFM)

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Super-Resolved Traction Force Microscopy (STFM)

Measuring small forces is a major challenge in cell biology. Here we improve the spatial resolution and accuracy of force reconstruction of the well-established technique of traction force microscopy (TFM) using STED microscopy. The increased spatial resolution of STED-TFM (STFM) allows a greater than 5-fold higher sampling of the forces generated by the cell than conventional TFM, accessing th...

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Native cell-material interactions occur on materials differing in their structural composition, chemistry, and physical compliance. While the last two decades have shown the importance of traction forces during cell-material interactions, they have been almost exclusively presented on purely elastic in vitro materials. Yet, most bodily tissue materials exhibit some level of viscoelasticity, whi...

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ژورنال

عنوان ژورنال: Nano Letters

سال: 2016

ISSN: 1530-6984,1530-6992

DOI: 10.1021/acs.nanolett.6b00273